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rat igg2a isotype control  (Bio X Cell)


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    Bio X Cell rat igg2a isotype control
    Rat Igg2a Isotype Control, supplied by Bio X Cell, used in various techniques. Bioz Stars score: 98/100, based on 1792 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rat+igg2a/InVivoMAb+rat+IgG2a+isotype+control%2C+anti-trinitrophenol/pmc13023788-248-20-29
    Average 98 stars, based on 1792 article reviews
    rat igg2a isotype control - by Bioz Stars, 2026-10
    98/100 stars

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    Control:

    Article Title: GFER Represents a Target for Dual Disruption of Redox Homeostasis and Reactivation of the Immune Response in Pancreatic Adenocarcinoma
    Article Snippet: 349 For in vivo CD8 depletion studies, anti-CD8 antibody (10mg/kg, # BE0117, clone YTS 169.4, 350 BioXcell) or rat IgG2a (#BE0089, BioXcell) treatment was administered to C57BL/6J male or 351 female mice one day before surgery and then administered intraperitoneally every 7 days post-352 surgery for 4 cycles. .. 354 For immune checkpoint blockade treatment, mice were intraperitoneally administered 100 μg of 355 anti-PD-1 antibody (#BE0146, clone RMP1-14, BioXcell) every 3 days for a total of 7 doses 356 since Day 1, with rat IgG2a (#BE0089, BioXcell) used as isotype control antibodies. .. 354 For immune checkpoint blockade treatment, mice were intraperitoneally administered 100 μg of 355 anti-PD-1 antibody (#BE0146, clone RMP1-14, BioXcell) every 3 days for a total of 7 doses 356 since Day 1, with rat IgG2a (#BE0089, BioXcell) used as isotype control antibodies.

    Article Title: PD-1 inhibitor improves radiosensitivity by tumor vessel normalization
    Article Snippet: .. For antibody depletion experiments, animals received 250 μg of depleting or isotype control monoclonal antibodies, including CD4 (clone YTS177, #BE0003-3, BioXCell), CD8 (clone 53-6.7, #BE0004-1, BioXCell), and rat IgG2a (clone 2A3, #BE0089, BioXCell) to deplete different T cell subsets. ..

    Article Title: PD-1 inhibitor improves radiosensitivity by tumor vessel normalization.
    Article Snippet: .. For antibody depletion experiments, animals received 250 μg of depleting or isotype control monoclonal antibodies, including CD4 (clone YTS177, #BE0003-3, BioXCell), CD8 (clone 53-6.7, #BE0004-1, BioXCell), and rat IgG2a (clone 2A3, #BE0089, BioXCell) to deplete different T cell subsets. ..

    Article Title: CEBPB Expression in Tumor Cells Drives Immune Evasion in Colorectal Cancer via CTLA4 Up-regulation in T Cells
    Article Snippet: When tumor volumes reached 75 to 125 mm 3 , the mice were randomized into 4 treatment groups: (a) anti-mouse CTLA-4 (BP0032, clone UC10-4F10-11, Bio X Cell), (b) anti-mouse PD-1 (BE0146, clone RMP1-14, Bio X Cell), (c) a combination of anti-CTLA-4 and anti-PD-1, and (d) control antibodies. .. Rat IgG2a (BE0089, Bio X Cell) and Armenian hamster IgG (BE0091, Bio X Cell) were used as isotype control antibodies. ..

    Article Title: CEBPB expression in tumor cells drives immune evasion in colorectal cancer via CTLA4 upregulation in T cells
    Article Snippet: When tumor volumes reached 75–125 mm3, the mice were randomized into four treatment groups: 1) antimouse CTLA-4 (BP0032, clone UC10-4F10-11, Bio X Cell), 2) anti-mouse PD-1 (BE0146, clone RMP1-14, Bio X Cell), 3) a combination of anti-CTLA-4 and antiPD-1, and 4) control antibodies. .. Rat IgG2a (BE0089, Bio X Cell) and Armenian hamster IgG (BE0091, Bio X Cell) were used as isotype control antibodies. ..

    Bioprocessing:

    Article Title: PD-1 inhibitor improves radiosensitivity by tumor vessel normalization
    Article Snippet: .. For antibody depletion experiments, animals received 250 μg of depleting or isotype control monoclonal antibodies, including CD4 (clone YTS177, #BE0003-3, BioXCell), CD8 (clone 53-6.7, #BE0004-1, BioXCell), and rat IgG2a (clone 2A3, #BE0089, BioXCell) to deplete different T cell subsets. ..

    Article Title: PD-1 inhibitor improves radiosensitivity by tumor vessel normalization.
    Article Snippet: .. For antibody depletion experiments, animals received 250 μg of depleting or isotype control monoclonal antibodies, including CD4 (clone YTS177, #BE0003-3, BioXCell), CD8 (clone 53-6.7, #BE0004-1, BioXCell), and rat IgG2a (clone 2A3, #BE0089, BioXCell) to deplete different T cell subsets. ..



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    CLCA4 suppressed colorectal cancer stem cell expansion by interacting with vimentin to suppress FAK signaling pathways. (A) Western blotting analysis of FAK and p-FAK protein levels in control and CLCA4-overexpressing colorectal cancer (CRC) cells. Right panels: Quantification of protein expression ratio. (B) Western blotting analysis of stemness-related proteins and p-FAK in CLCA4-overexpressing cells treated with or without FAK agonist. Lower panels: Quantification of protein expression ratio. (C) Tumorsphere formation assay was performed to examine the tumorsphere formation ability in CLCA4-overexpressing cells treated with or without FAK agonist. One-way ANOVA with Tukey's multiple comparisons test (mean ± standard deviation). (D) Immunoprecipitation and <t>IgG</t> samples were analyzed by mass spectrometry. Proteins with unused >1.3 were filtered out, and keratin was removed. A total of 336 proteins were identified, including 334 proteins in immunoprecipitation samples and 4 proteins in IgG samples. (E) The immunoprecipitates of CLCA4 were purified using anti-Flag antibody and separated with SDS-PAGE, and the presence of vimentin was analyzed by Western blotting. Normal IgG was used as the negative control. (F) The immunoprecipitates of vimentin were purified using anti-HA antibody and separated with SDS-PAGE, and the presence of CLCA4 was analyzed by Western blotting. Normal IgG was used as the negative control. (G) The differences in protein levels (vimentin, Bmi-1, and p-FAK) among CRC cells transfected with different plasmids were analyzed by Western blotting. Right panels: Quantification of protein expression ratio. (H) Tumorsphere formation assay was performed to examine the tumorsphere formation ability among CRC cells transfected with different plasmids. One-way ANOVA with Tukey's multiple comparisons test (mean ± standard deviation).
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    Image Search Results


    CLCA4 suppressed colorectal cancer stem cell expansion by interacting with vimentin to suppress FAK signaling pathways. (A) Western blotting analysis of FAK and p-FAK protein levels in control and CLCA4-overexpressing colorectal cancer (CRC) cells. Right panels: Quantification of protein expression ratio. (B) Western blotting analysis of stemness-related proteins and p-FAK in CLCA4-overexpressing cells treated with or without FAK agonist. Lower panels: Quantification of protein expression ratio. (C) Tumorsphere formation assay was performed to examine the tumorsphere formation ability in CLCA4-overexpressing cells treated with or without FAK agonist. One-way ANOVA with Tukey's multiple comparisons test (mean ± standard deviation). (D) Immunoprecipitation and IgG samples were analyzed by mass spectrometry. Proteins with unused >1.3 were filtered out, and keratin was removed. A total of 336 proteins were identified, including 334 proteins in immunoprecipitation samples and 4 proteins in IgG samples. (E) The immunoprecipitates of CLCA4 were purified using anti-Flag antibody and separated with SDS-PAGE, and the presence of vimentin was analyzed by Western blotting. Normal IgG was used as the negative control. (F) The immunoprecipitates of vimentin were purified using anti-HA antibody and separated with SDS-PAGE, and the presence of CLCA4 was analyzed by Western blotting. Normal IgG was used as the negative control. (G) The differences in protein levels (vimentin, Bmi-1, and p-FAK) among CRC cells transfected with different plasmids were analyzed by Western blotting. Right panels: Quantification of protein expression ratio. (H) Tumorsphere formation assay was performed to examine the tumorsphere formation ability among CRC cells transfected with different plasmids. One-way ANOVA with Tukey's multiple comparisons test (mean ± standard deviation).

    Journal: Genes & Diseases

    Article Title: Chloride channel accessory 4 suppresses stem cell-like properties of colorectal cancer and enhances anti-PD-1 immunotherapy

    doi: 10.1016/j.gendis.2025.101859

    Figure Lengend Snippet: CLCA4 suppressed colorectal cancer stem cell expansion by interacting with vimentin to suppress FAK signaling pathways. (A) Western blotting analysis of FAK and p-FAK protein levels in control and CLCA4-overexpressing colorectal cancer (CRC) cells. Right panels: Quantification of protein expression ratio. (B) Western blotting analysis of stemness-related proteins and p-FAK in CLCA4-overexpressing cells treated with or without FAK agonist. Lower panels: Quantification of protein expression ratio. (C) Tumorsphere formation assay was performed to examine the tumorsphere formation ability in CLCA4-overexpressing cells treated with or without FAK agonist. One-way ANOVA with Tukey's multiple comparisons test (mean ± standard deviation). (D) Immunoprecipitation and IgG samples were analyzed by mass spectrometry. Proteins with unused >1.3 were filtered out, and keratin was removed. A total of 336 proteins were identified, including 334 proteins in immunoprecipitation samples and 4 proteins in IgG samples. (E) The immunoprecipitates of CLCA4 were purified using anti-Flag antibody and separated with SDS-PAGE, and the presence of vimentin was analyzed by Western blotting. Normal IgG was used as the negative control. (F) The immunoprecipitates of vimentin were purified using anti-HA antibody and separated with SDS-PAGE, and the presence of CLCA4 was analyzed by Western blotting. Normal IgG was used as the negative control. (G) The differences in protein levels (vimentin, Bmi-1, and p-FAK) among CRC cells transfected with different plasmids were analyzed by Western blotting. Right panels: Quantification of protein expression ratio. (H) Tumorsphere formation assay was performed to examine the tumorsphere formation ability among CRC cells transfected with different plasmids. One-way ANOVA with Tukey's multiple comparisons test (mean ± standard deviation).

    Article Snippet: After 7 days, mice were intraperitoneally treated with either an in vivo blocking antibody against mouse PD-1 (Clone: 29F.1A2, BioXcell, Cat# BP0273) or a rat IgG2a isotype control antibody (Clone: 2A3, BioXcell, Cat# BP0089).

    Techniques: Protein-Protein interactions, Western Blot, Control, Expressing, Tube Formation Assay, Standard Deviation, Immunoprecipitation, Mass Spectrometry, Purification, SDS Page, Negative Control, Transfection